A method for fixing and paraffin embedding tissue to retain the natural fluorescence of reporter proteins
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چکیده
منابع مشابه
A method for fixing and paraffin embedding tissue to retain the natural fluorescence of reporter proteins.
Green fluorescent protein (GFP) and its derivatives are routinely employed as surrogate markers for gene expression and lineage tracing in genetically engineered mice. Tissues from these mice are commonly formalin fixed and paraffin embedded (FFPE) for histological studies. However, this results in inactivation of the natural fluorescence of these proteins, requiring their detection by immunolo...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2015
ISSN: 1940-9818,0736-6205
DOI: 10.2144/000114328